Table 1 Primers for qPCR
Target genes | Primer pairs(5’ to 3’) | |
|---|---|---|
Forward | Reverse | |
PI3K | GCCGATCCTACAGTCCTATCCAATG | GCAGAAGGCACAGGTCCAGAG |
Akt | AGGAGGAGGAGACGATGGACTTC | ACACGGTGCTTGGGCTTGG |
Bcl-2 | TGGAGAGCGTCAACAGGGAGATG | GGTGTGCAGATGCCGGTTCAG |
Bax | GATGCGTCCACCAAGAAGCTGAG | CACGGCGGCAATCATCCTCTG |
IL-6 | ACTTCCAGCCAGTTGCCTTCTTG | TGGTCTGTTGTGGGTGGTATCCTC |
IL-10 | CTGCTCTTACTGGCTGGAGTGAAG | TGGGTCTGGCTGACTGGGAAG |
ZO-1 | GCCAAGCCAGTCCATTCTCAGAG | TCCATAGCATCAGTTTCGGGTTTCC |
Occludin | CAACGGCAAAGTGAATGGCAAGAG | TCATCCACGGACAAGGTCAGAGG |
Claudin-1 | CTTCTGGGTTTCATCCTGGCTTCG | CCTGAGCAGTCACGATGTTGTCC |
Ghrelin | ACCAGAAAGCCCAGCAGAGAAAG | CGAAGGGAGCATTGAACCTGATTTC |
PYY | GGAGCTGAGCCGCTACTATGC | AGATTCTCGCTGTCGTCTGTGAAG |
β-actin | ACCGTGAAAAGATGACCCAGCAT | CCAGAGGCATACAGGGACAA |