Changes in gut microbiota following supplementation with chitosan in adolescents with overweight or obesity: a randomized, double-blind clinical trial

Table 1 Specifications of the primers used for each bacterial strain

Number	Primer	Sequence 5'→ 3'	Length	Tm
1	Bifidobacterium [26–28]	FP: GCGTGCTTAACACATGCAAGTC	22	59
2	Bifidobacterium [26–28]	RP: CACCCGTTTCCAGGAGCTATT	22	59
3	Firmicuits [29]	FP:GGAGCATGTGGTTTAATTCGAAGCA	25	56.6
4	Firmicuits [29]	RP: AGCTGACGACAACCATGCAC	21	57.3
5	Bacteroidetes [29]	FP: GGAACATGTGGTTTAATTCGATGAT	25	59.8
6	Bacteroidetes [29]	RP: AGCTGACGACAACCATGCAG	21	60
7	Lactobacillus [27]	FP: TACATCCCAACTCCAGAACG	20	56.4
8	Lactobacillus [27]	RP: AAGCAACAGTACCACGACCA	21	58.4
9	Akkermansia [29]	FP:CAGCACGTGAAGGTGGGGAC	20	59.1
10	Akkermansia [29]	RP:CCTTGCGGTTGGCTTCAGAT	21	59.4

ISSN: 1758-5996