Fig. 3

Acetylome analysis. (3 A) 86 downregulated and 61 upregulated Kac sites in VA compared to SA. Correlation analysis was shown in the heatmap dia gram. (3B) The top 5 upregulated (red) and downregulated (blue) Kac sites in VA compared to SA were shown in the volcano plot. (3 C) The top 10 upregulated (red) and downregulated (blue) Kac sites were shown in the radar plot, with the second ring showed the log2-transformed FC values, and the third ring represented the average quantification levels. (3D) GO enrichment for BP, CC, and MF categories, selecting the top 5 upregulated (red) and downregulated (blue) Kac sites in VA compared to SA for each category, longer bars indicated smaller p-values while larger points represented more genes. (3E) KEGG enrichment bubble chart (right) combined with a Sankey-bubble plot (left). The Sankey plot represented acetylated proteins included in each pathway, while the bubble plot displayed bubble size as the number of acetylated proteins in the pathways and bubble color as the p-value. The top 10 upregulated and downregulated pathways were selected for visualization