Fig. 1

Transcriptomic analysis of serum from subjects. A The schematic diagram depicts the recruitment of both normal individuals and O-T2DM patients. B, C Visual statistical results of differential gene analysis. Volcano plots of mRNA (B), circRNA (C) expression. Each dot in the graph represents a specific gene or transcript; the red dots represent significantly upregulated genes; the green dots represent significantly downregulated genes, and the gray dots represent genes without significantly different expression. D The relationship between the mean connectivity and various soft-thresholding powers. E The relationship between the scale-free fit index and various soft-thresholding powers. F Clustering dendrogram of 30 samples. G The cluster dendrogram was constructed using WGCNA based on topological overlap dissimilarity, with each distinct color denoting a particular gene co-expression module. H Heat map of the correlation between each color module and phenotype