Fig. 2

ADSC/GDM-derived inhibits hepatocyte proliferation and insulin sensitivity in normal mice. A, B The sEV^A and sEV^AG with different concentrations were co-cultured with AML12 cells for 48 or 72 h. Cell viability was obtained by CCK8 assays. C, D The sEV^A and sEV^AG with 10⁸ particles / mL were co-cultured with AML12 cells for 48 or 72 h. Cell apoptosis was detected by flow cytometry. E, F The sEV^A and sEV^AG with 10⁸ particle number / mL were co-cultured with AML12 cells for 48 or 72 h, then cells were treated with 1 μg/ml insulin for 1 h. Glucose uptake was measured to reflect insulin sensitivity of AML12 cells in each treatment. G The sEV^A and sEV^AG with 10.⁸ particle number /mL were co-cultured with AML12 cells for 48 h, and mRNA expression of endoplasmic reticulum (ER)-related genes was detected by RT-PCR. H The expression of ER-related proteins was detected by western blotting. Data were presented with mean ± standard deviation (SD); ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001